MCDh (Micro Chromatic Detection for Haematology) is a panoptic haematology staining method. Methanol-free, it is made of a sequence of four specific reagents used in blood and bone marrow cell differentiation and counting. This new generation of stains is in line with the standardisation trend in diagnostic techniques.
Ethanol-based, MCDh 1 is a blend of neutral stains used as a fixation and set-up solution, particularly for the water-soluble elements (basophilic granules). These stains are not active in alcoholic media and only act selectively when released into MCDh 2. This leads to the precipitation of stains in erythrocytes, eosinophil granules and neutrophil leukocyte cytoplasma. MCDh 3 is used for monocyte and lymphocyte cytoplasm staining and red metachromatic staining of azurophil granules. MCDh 4 will remove excess stain and complete the staining.
Standard Procedure for MCDh Blood Smear
Reagent | Size | Product Code |
MCDh 1 | 1ℓ / 2.5ℓ | CB313590 - 1000/2500 |
MCDh 2 | 1ℓ / 2.5ℓ | CB313570 - 1000/2500 |
MCDh 3 | 1ℓ / 2.5ℓ | CB313560 - 1000/2500 |
MCDh 4 | 1ℓ / 2.5ℓ | CB313600 - 1000/2500 |