recomBlot Rubella IgG CB35/4902
RecomBlot
Qualitative in vitro test for the indirect detection and safe identification of pathogen specific antibodies. As a western blot the recomBlot serves to confirm the results from screening assays such as enzyme immuno assays (ELISA) or immunofluorescence assays (IFA). In contrast to ordinary immunoblot assays, the antigens are presented optimal in the recomBlot without disturbing and cross-reacting proteins. For this reason the recomBlot displays a high specificity and sensitivity. Furthermore the recomBlot is easy and secure to read
Immunoblot test with antigens produced by recombinant techniques for the detection of IgG antibodies against the Rubella virus. Differentiation test between rubella immunity and rubella primary infection.
In spite of the availability of a protective inoculation against the Rubella virus, about 200 children with rubella syndrome are born in Germany every year. Therefore, it is of great importance that the immune status for the rubella virus is determined and monitored before and during pregnancy.
Problems can arise in the serological differentiation between past infection or inoculation and an acute primary infection. The reasons for this are unclear IgG results and especially weak IgM titres, which make the exclusion of a fresh infection difficult.
recomBlot Rubella IgG was developed for these diagnostic situations. The test uses recombinant Rubella specific structural proteins which are separated electrophoretically and transferred to a nitrocellulose membrane (Western blot). Thus, an evaluation of reactivities with individual antigens is possible.
As a rule, IgG antibodies against the E2 antigen are found months after primary infection. Consequently, the determination of this antibody specificity can provide additional information for the evaluation of doubtful serological results.