Product Information

recomBlot EBV IgG   CB53/4502

recomBlot EBV IgM/IgA   CB53/4503

 

RecomBlot

Qualitative in vitro test for the indirect detection and safe identification of pathogen specific antibodies. As a western blot the recomBlot serves to confirm the results from screening assays such as enzyme immuno assays (ELISA) or immunofluorescence assays (IFA). In contrast to ordinary immunoblot assays, the antigens are presented optimal in the recomBlot without disturbing and cross-reacting proteins. For this reason the recomBlot displays a high specificity and sensitivity. Furthermore the recomBlot is easy and secure to read

 

Immunoblot test with antigens produced by recombinant techniques for the detection of IgG, IgM and IgA antibodies against the Epstein-Barr virus (EBV).

 

The Epstein-Barr virus, a ubiquitously occurring herpes virus, can cause the symptoms of infectious mononucleosis (Pfeiffer’s disease) on primary infection. Moreover, as a result of the lifelong persistence of this pathogen, reactivations can occur, especially in immuno-incompetent persons.

 

Due to the diversity of symptoms caused by primary infection or reactivation and their correspondence with the symptoms of other diseases, one of the main tasks in routine diagnosis is the serological detection of a primary infection, past infection or possible reactivation. For this purpose, a series of individual determinations (EIA and IFT) are generally carried out for the particular class of antigen and type of antibody.

 

The Western blot technique allows, at a glance, the detection and identification of IgG, IgM, and IgA antibodies against various classes of antigens. The application of highly specific and characteristic EBV proteins is made possible by the use of antigens produced by genetic engineering.

 

Here the antigens EBNA-1 and p18 are of major importance: Due to the fact, that anti- EBNA-1 and/or anti-p18-IgG antibodies are detected only in the case of post acute or past EBV infections (s. Evaluation), more than 95 % of the past EBV infections can be correctly identified with the recomBlot EBV IgG strip only.