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Tristel Duo Sporocidal Disinfectant

 

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Evaluation/validation documents

Tristel DUO sporicidal surface disinfectant

CB83/DUO/LAB

2 aliquots of foam cover 60cm2 surface. Approx. 140 aliquots per container

Validation of Tristel Duo’s efficacy against B. subtilis spores suspension test

Validation of Tristel Duo’s efficacy against B. subtilis  surface test

Validation of Tristel Duo’s efficacy against C.difficile surface test

Validation of Tristel Duo’s Mycobactericidal activity suspension test

Evaluation of the Bactericidal activity of Tristel Duo surface tests

Evaluation of the virucidal activity of Tristel Duo against Human Norovirus (Feline calicivirus surrogate) and SARS virus (Feline coronavirus surrogate)

Cosmos Biomedical Ltd has exclusive UK distribution rights for sale of Tristel products into research, veterinary and food and water laboratories. 

 

Byocor™ Antimicrobial Wipe

 

  • Incorporates Byotrol™ - a newly available biocide tested to EN1276, EN13704 & EN1650 (what is Byotrol™?)
  • Kills bacteria (including E. coli, MRSA & Clostridium difficile), fungi, moulds and viruses
  • Residual activity - continues working and protecting days after it has dried
  • Unique technology prevents microbe resistance
  • Safe to use on all non-porous surfaces
  • No alcohol added - gentle on hands and skin
  • Each wipe cleans and protects an area of 4 square metres

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    Product

    Product Code

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    Press Release

    Byocor Antimicrobial Wipe impregnated with Byotrol™

    CB83/100

    1 wipe per sealed sachet

    read article

     

    Directions for use:

    • Tear open the sachet at the notch and remove wipe
    • Suitable for use on most hard surfaces including wood, glass, plastic and painted and unpainted metals
    • Byocor wipes can also be used as a sanitising wipe for hands and unbroken adult skin
    • As with all cleaning products, keep out of reach of children
    • After use, dispose of thoroughly with all packaging

    What is Byotrol™?

    Byotrol™ possesses a number of characteristics rendering it an ideal antimicrobial agent. Byotrol™ possesses the following characteristics:

    • Durable and long lasting activity - effective days to months even after it dries.
    • Broadband efficacy including 'superbugs' - effective against pathogens such as MRSA, VRE, C. difficile, E. coli, algae, fungi and viruses.
    • Developed resistance extremely unlikely - the technology affects all microscopic organisms, physically damaging them and rendering all vulnerable to the chemical controls.
    • Low toxicity - not considered poisonous to humans, animals or plants.
    • Easy to use - simple application.
    • Environmentally friendly - fully biodegradable –breaks down when in landfill / waste water.
    • Byotrol does not contain antibiotics over use of which can lead to resistance, heavy metals (mercury and lead) which are poisons, toxic metal salts (tin and copper etc.), Poly Chlorinated Bi-Phenols.

     

     

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    Tristel DUO - Evaluation and Validation Information

    Validation of Tristel Duo’s efficacy against Bacillus subtilis spores – suspension test

    Objective:
    To assess the sporicidal activity of Tristel chlorine dioxide solution present in a foam system using a suspension test, in the presence and absence of an organic load.

    Methodology:
    European Phase 2 tests to establish sporicidal activity have not yet been agreed. The suspension test used was first described by Babb JR, Bradley CR, Ayliffe GAJ (1990) sporicidal activity of glutaraldehydes and hypochlorites and other factors influencing their selection for the treatment of medical equipment. Journal of Hospital Infection 1: 63-76.

    Test organism:
    Bacillus subtilis var niger NCTC 10073 (UK chemical sterilisation validation strain).

    Results:

    Sporicidal activity of Tristel (diluted 1 in 10) Log10 reductions obtained after specified times

         
    Contact times Clean Conditions Dirty Conditions
    0 mins (challenge) 7.72 7.66
    30 secs >6.72 >6.66
    1 min >6.72 >6.66
    2 mins >6.72 >6.66
    5 mins >6.72 >6.66

    Interpretation:
    A >6 log10 reduction is used as an indication of acceptable sporicidal activity. Tristel foam solution tested freshly prepared in a 1 in 10 dilution of the use concentration gave a >6 log10 reduction in 30 seconds under clean and dirty conditions.

    Data source:
    Hospital Infection Research Laboratory, Birmingham, February 2005

     

    Validation of Tristel Duo’s efficacy against Bacillus subtilis var niger NCTC 10073 – surface test   

    Methodology:

    A 12 inch square test surface was marked out on a stainless steel plate. The test surface was inoculated with 1.0ml of aqueous suspension with 107 spores and the inoculum was allowed to dry out naturally at room temperature for 30 minutes.

    The Tristel Duo Foam was applied to the test surface (three aliquots) and dispersed with the Tristel Duo Wipe for 15 seconds (activation/spreading stage) and then the test surface was systematically wiped for a further 30 seconds (disinfection stage). Suspensions were taken from the surface of the test site and from the wipe itself.

    Results:

    Inoculum
    (spores)    		 Disinfectant
    		 Activation
    time (secs)    		 Wipe time
    (secs)    		 Viable count
    surface    		 viable count
    wipe
    107 Tristel Duo 15 30 0 0

    Interpretation:
    Three aliquots of Tristel Duo Foam dispersed with a Tristel Duo Wipe achieved a 100% kill of the Bacillus subtilis spores (approximately 107) dried out on a 12 inch square stainless steel test surface.

    Data source:
    Dr David Coates, March 2005

     

    Validation of Tristel Duo’s efficacy against Clostridium difficile ATCC 9689 – surface test

    Methodology:

    The test organism was inoculated into 9ml of cooked meat medium (Biomerieux) and incubated at 370C for 48 hours to obtain a culture containing approximately 108 cells/ml (actual count = 1.1 x 108/ml).  A 12 inch square test surface was marked out on a stainless steel plate and one ml of inoculum was spread over the test surface and allowed to dry for 30 minutes.  The Tristel Duo was wiped systematically over the test surface for 30 seconds.Suspensions were taken from the surface of the test site and from the wipe itself, plated out on HBA plates and incubated in an anaerobic jar for five days at 37oC .

    Results:

    Inoculum
    (spores)    		 Disinfectant
    		 Activation
    time (secs)    		 Wipe time
    (secs)    		 Viable count
    surface
    108 Tristel Duo 30 0 0

    Interpretation:
    Tristel Duo Wipe achieved a 100% kill of vegetative cells of Clostridium difficile ATCC 9689 (1.1 x 107) dried out on a 12 inch square stainless steel test surface. (Wipe time: 30 seconds)

    Data source:
    Dr David Coates, April 2005

     

    Validation of Tristel Duo’s Mycobactericidal activity – suspension test   

    Objective:
    To assess the mycobactericidal activity of Tristel chlorine dioxide solution present in a foam system using a suspension test, in the presence and absence of an organic load.

    Methodology:
    The European test method to establish tuberculocidal activity has not yet been formulated and ratified. The test method used is that of Griffiths et al., Journal of Hospital Infection (1998) 38: 183 – 192.

    Test organism:
    Mycobacterium terrae NCTC 10856 (ATCC 15755) was used as the test organism to represent tuberculocidal activity. It is now recognised that this organism is a suitable surrogate test organism for Mycobacterium tuberculosis and can be used to establish tuberculocidal activity (CEN TC 216).

    Results:

    Mycobactericidal activity of Tristel (diluted 1 in 10) Log10 reductions obtained after specified contact times
    Contact time Clean conditions Dirty conditions
    0 mins challenge   8.82   8.69
    30 secs >5.82 >5.69
    1 min >5.82 >5.69
    2 mins >5.82 >5.69
    5 mins >5.82 >5.69

    Interpretation:
    A > 5 log10 reduction in Mycobacterium terrae is used as an indication of high/intermediate level disinfection and tuberculocidal activity. Tristel foam solution tested freshly prepared in a 1 in 10 dilution of the use concentration gave a >5log10 reduction in 30 seconds under clean and dirty conditions when freshly prepared.

    Data source:
    Hospital Infection Research Laboratory, Birmingham, February 2005

     

    Evaluation of the Bactericidal activity of Tristel Duo – surface tests

    Objective & Methodology:

    The bactericidal activity of the Tristel Duo wipe was assessed against Pseudomonas aeruginosa NCTC 6749, Acinetobacter baumanii NCTC 10303, Escherichia coli NCTC 8196 and Staphylococcus aureus NCTC 4163, using the standard surface test methodology described above.

    Results:

      Pseudomonas
    aeruginosa    		 Acinetobacter
    baumanii    		 Escherichia
    coli    		 Staphylococcus
    aureus
    Test surface
    area    		 12" sq 12" sq 12" sq 12" sq
    Contact
    time    		 30 secs 30 secs 30 secs 30 secs
    Initial count > 108 > 108 > 108 > 108
    Viable count
    surface    		 0 0 0 0
    Viable count wipe 0 0 0 0

    Interpretation:
    Tristel Duo Wipe was completely effective in killing all organisms on both the test surface and the wipe with a 30 second contact time.

     

    Evaluation of the virucidal activity of Tristel Duo against Human Norovirus (Feline calicivirus as surrogate) and SARS virus (Feline coronavirus as surrogate)   

    No cell culture system currently exists for human noroviruses. The surrogate challenge virus for Human Norovirus, Feline calicivirus (FCV) 62804, was a local isolate from the Department of Veterinary Pathology, University of Glasgow Vet School. No cell culture propagation systems are currently available for SARS virus that do not require the highest levels of containment. Feline coronavirus (FCoV) is structurally and biologically similar to the SARS virus, and, therefore, FCoV is a suitable surrogate. FCV and FCoV give a distinct cytopathic effect in feline fibroplasts (FEAs – Feline embryo A) within three days of inoculation by the virus. Several ten-fold dilutions of the virus prior to inoculation of FEAs can be used to quantify viable virus recovery following treatment of the target virus. The target virus is treated with the disinfectant (test substance) under laboratory conditions.

    Feline coronavirus suspension test results for the efficacy of Tristel Duo solution at a 30 seconds contact with a soil load of 0.6 G/L as foetal bovine serum

    30 secs contact  Virus recovery (TCID 50/ml) Cytotoxicity (80% disinfectant)
    (TCID 50/ml)    		 5.0% Disinfectant (TCID 50/ml)
    1     <7.9x102
    2     <7.9x102
    3     <7.9x102
    4     <7.9x102
    Mean <7.3x105 <7.9x103 <7.9x102
    Log 5.9   <2.9
    Log diffence     >3.0

     

    Feline calicivirus suspension test results for the efficacy of Tristel Duo solution at a 30 seconds contact with a soil load of 0.6 G/L as foetal bovine serum
    30 secs contact Virus recovery (TCID 50/ml) Cytotoxicity (80% disinfectant) 80.0% Disinfectant
    (TCID 50/ml)
    1     1.3x104
    2     5.0x104
    3     <8.0x103
    4     5.0x107
    Mean 4.9x107 <2.5x103 1.2x104
    Log 7.7   4.1
    Log difference     3.6

    Interpretation:

    SARS virus (FCoV as surrogate)
    The result at a 5% v/v concentration of agent indicates that the working concentration of this agent (neat) would be expected to effectively reduce the infectivity of SARS virus within 30 seconds of contact.

    Human norovirus (FCV as surrogate)
    The result indicates that the working concentration (neat) of the agent would effectively reduce Human Norovirus infectivity within 30 seconds of contact.

    Data source:
    Dr Chris Woodall, Blu Scientific Test Data, School of Life Sciences, Glasgow Caledonian University, May 2005.

     

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