Product Information

recomBlot Treponema IgG   CB37/5102

recomBlot Treponema IgM   CB37/5103

 

Immunoblot test with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Treponema pallidum.

 

Treponema pallidum subsp. pallidum is the causative agent of Syphilis, a chronic disease occurring only in humans. The disease progresses through stages. After a short propagation period at the site of infection (primary stage) the hematogenic spreading of the pathogen throughout the entire organism follows (secondary stage). After the decline of acute symptoms Treponema pallidum can persist for years or lifetime in the human organism (stage of latency) and then can cause late complications (tertiary Syphilis). During pregnancy the pathogen can be transmitted to the foetus (Syphilis connata). In approximately 40 % of the cases infection results in abortion or death of the child shortly after birth. The probability of infection in utero is around 40-70 %.

 

The serologic diagnosis of syphilis intends the use of a sensitive antibody-screening assay in the first step (TPHA, ELISA). In the case of equivocal or positive results in the screening test a confirmation assay is performed in the second step to assure the specificity (FTA-ABS, immunoblot). Here the immunoblot plays a very important role, as only with the western blot technique the determination of antibody production against essential Treponema pallidum antigens is possible. At the same time the detection of different antibody classes is possible. With the use of recombinant antigens the recomBlot Treponema IgG/IgM meets all these requirements perfectly.