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DEXTROSE TRYPTONE BROTH

 

A general purpose liquid medium.

 

Code: CB1/5862

 

Typical formula g/l

 

Beef extract

3.0

Tryptose 10.0 
Glucose 10.0
Sodium chloride 5.0

  

Final pH 7.2 +/- 0.2   

 

Directions

Suspend 43g of Dextrose Agar or 23g of Dextrose Broth in 1000ml of cold distil­led water, heat to boiling, distribute and autoclave at 121°C for 15 minutes.

 

Description

Dextrose Agar and Broth are general purposes media, used for the cultivation of a large variety of microorganisms, including fastidious types, such as streptococci, meningococci and pneumococci. The glucose incorporated in the medium, being rapidly metaboli­sed by many microorganisms, supports good microbial growth. Dextrose Agar can be used as it is, with the addition of 5% of sterile defibrinated blood for the preparation of blood agar, or heated to 80°C for the preparation of chocolate agar. The medium is not suitable for demonstrating the haemolytic properties of microorganisms, due to the high glucose content. With the addition of 0.1% agar, Dextrose Broth supports a more rapid and luxuriant growth of pathogenic cocci (pneumococci, meningococci, etc.).

 

User quality assurance (37°C-24 h)

 

Productivity control

S.aureus ATCC 25923: growth

E.coli ATCC 25922: growth

 

Storage

Dehydrated media: 15-30°C

User prepared media: 1 month at 2-8°C

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