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D.C.L.S. Agar (Desoxycholate Citrate Lactose Sucrose Agar)

A modification of D.C.A. medium for the improved isolation and recognition of Salmonella and Shigella spp.

Code: CB1/1025

Typical formula g/l

Peptone mix

10.0

Beef extract

5.0

Lactose

 5.0
Sucrose 5.0
Sodium citrate 7.5
Sodium thiosulphate

2.5

Sodium desoxycholate

2.5

Agar 12.0
Neutral red 0.03

 pH: 7.2 ± 0.2

Directions:

Suspend 50 grams of powder, disperse in 1 litre of deionised water. Allow to stand for 10 minutes then heat gently with frequent mixing and bring to the boil. Simmer for 1 minute to complete dissolution of the solids. Cool to 47șC then distribute 20ml into Petri dishes. Dry the surface by partial exposure, before use. Do not re-melt or autoclave this medium.

Q.C. organisms: Salmonella spp. , E.coli

Storage: Plates up to 7 days at 2-8șC in the dark.

Inoculation: Surface plating, streaking out to single colonies.

Incubation: 37șC aerobically for 24 hours.

References:

Hynes, M. J Path Bact. 1942. 54: 193-207

Leifson, E. J Path Bact 1935. 40: 581-589.

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