CZAPEK DOX BROTH

Used for the cultivation of saprophytic fungi.

Code: CB1/6456

Typical formula g/l

Sucrose	30.00
Sodium nitrate	2.00
Potassium chloride	0.50
Magnesium glycerophosphate	0.50
Ferrous sulphate	0.01
Potassium sulphate	0.35

pH 7.0 +/- 0.2

Directions

Suspend 49g of agar and 35.4g of broth in 1000ml of cold distilled water. Heat to boiling, distribute and autoclave at 121^{_{°C for 15 minutes.}}

_Description

_{Czapek Dox Broth is a semi-synthetic medium of defined chemical composition used for the cultivation of saprophytic fungi and bacteria able to use inorganic nitrogen.}

_{The sole source of inorganic nitrogen in the two media is the sodium nitrate, and the sole carbon source is the sucrose. Czapek Dox Broth is especially suitable for the enrichment, cultivation and identification of bacteria and fungi present in soil.}

_{User quality assurance (25°C-72 h)}

_{Productivity control}

_{C.albicans ATCC 18804: growth}

_{Asp.niger ATCC 9642: growth}

_Storage

_{Dehydrated media: 15-30°C}

_References

_{Booth, C. (1971) - Methods in Microbiology Vol. 4, London: Academic Press.}

_{Dox, (1910) - U.S. Dept. Ag. Anim. Ind. Bull.: 120, 70.}