[an error occurred while processing this directive] [an error occurred while processing this directive] [an error occurred while processing this directive]

CLED MEDIUM (SINGLE INDICATOR)

 

A non-inhibitory medium for the total microbial count in urine tract infections.

 

Code: CB1/1018

Typical formula g/l

Peptone 7.00
Tryptone 4.00
Lactose 10.00
L-Cysteine 0.128
Bromothymol blue 0.020
Agar

15.00

    

Final pH 7.3 +/- 0.2

 

Directions

Suspend 36g in 1000 ml of cold distilled water. Heat to boiling and autoclave at 121°C for 15 minutes.

 

Description

Cystine Lactose Electrolyte Deficient (CLED) Medium, prepared according to a modification of the original formula of Sandy’s, is a non-inhibitory medium particularly recommended for the total microbial count in urine. The medium supports the optimal growth of urinary pathogens, and provides a clear differentiation of the colonies. The lactose is present in the medium as a fermentable carbohy­drate; lactose-fermenting bacteria acidify the medium with a co­lour change of the indicator (Bromothymol Blue) to yellow. CLED Medium is deprived of electrolytes to prevent swarming of Proteus spp. The medium is used onto plates, bi and tri-plates or on dip-slides, coupled with MacConkey Agar for rapid evaluation of the number of bacteria in the urine.

 

User quality assurance   (37°C-24 h)

 

Productivity control

E. coli ATCC 25922: growth, yellow colonies

S. aureus ATCC 25923: growth, uniform deep yellow colonies

P. vulgaris ATCC 8427:growth, bluish colonies, spreading inhibited

 

Storage

Dehydrated medium: 15-30°C

User prepared plates: 1month at 2-8°C

 

References  

Mackey J. P. & Sandy’s, G.H. (1960’s) J.Med. Lab. Techn. 17 224 

[an error occurred while processing this directive]