CHROMOSENSE CANDIDA ALBICANS AGAR
A chromogenic selective and differential medium for the isolation of Candida spp. and the immediate identification of C. albicans.
Code: CB1/205
Typical formula g/l
| Growths Factors | 18.50 |
| Chloramphenicol | 0.05 |
| Gentamicin | 0.10 |
| Tryptone | 20.00 |
| Glucose | 1.00 |
| Agar | 13.00 |
| Chromogenic substrate |
0.10 |
pH: 6.3 +/- 0.2
Directions
Suspend 52.75 in 1000ml of cold distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 15 minutes. Cool to 50°C and distribute into sterile petri dishes.
Description
Chromogenic Candida albicans Agar is a selective and differential medium for the isolation of Candida spp. and the immediate identification of C. albicans. The selectivity of the medium is obtained by the presence of chloramphenicol and gentamicin. The differentiation is obtained by the presence of a chromogenic substrate in the medium to detect a specific enzymatic activity (beta-glucosaminidase) of C. albicans.
Directions
Suspend 52.75 in 1000ml of cold distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 15 minutes. Cool to 50°C and distribute into sterile petri dishes. Final pH: 6.3 +/- 0.2
Method
The medium can be employed according to usual laboratory techniques. C. albicans grows with blue or green-blue colonies after incubation at 37°C for 24 - 48 hours. Other species of the Candida genus grow with colourless colonies
Quality Control (25°C-72 hrs)
Productivity control
C.albicans ATCC 18804: growth
Selectivity Control
E.coli ATCC 25922: inhibited
Storage
Dehydrated medium: 2-8°C
User prepared plates: 7days at 2-8°C