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CETRIMIDE AGAR (U.S.P.)

Used for the isolation of Pseudomonas aeruginosa from pharmacological preparations.

Code: CB1/1202

Typical formula g/l

Gelatin peptone

20.0

Magnesium chloride 1.4
Potassium sulphate

10.0

Cetrimide 0.3
Agar 13.6

pH: 7.2 ± 0.2

Directions

Weigh 45.3 grams of powder, disperse in 1 litre of deionised water. Add 10mls of glycerol, allow to soak for 10 minutes then swirl to mix. Sterilise at 121șC for 10 minutes.

Description

A medium recommended by the United States Pharmacopoeia for the isolation of Pseudomonas aeruginosa from pharmacological preparations. Subculture is carried out onto the medium after enrichment in Tryptone Soy Broth. Cetrimide inhibits the growth of many microrganisms whilst allowing Ps. aeruginosa to develop typical colonies, which will fluoresce in ultraviolet light and produce green pigment.

Appearance: Opalescent, pale yellow agar.

Q.C. organisms: Ps. aeruginosa , E. coli (inhibition)

Storage: Plates up to 7 days at 2-8șC in the dark.

Inoculation: Subculture from enrichment broth, streak out for single colonies.

Incubation: 30-35șC aerobically for 24-48 hours.

References

United States Pharmacopoeia XXI. 1985.

Brown V. I., Lowbury E. J. L. 1965. Use of an improved Cetrimide Agar Medium and other culture methods for Pseudomonas aeruginosa. J. Clin. Pathol. 18, 752-756.

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