[an error occurred while processing this directive]CONTAGIOUS EQUINE METRITIS ORGANISM (C.E.M.O.) AGAR BASE
A selective isolation medium for Taylorella equigenitalis the causative organism of Contagious Equine Metritis.
Code: CB1/2204
Typical formula g/l
Tryptone 15.0 Soy peptone 5.0
Sodium chloride 5.0 Agar 12.0
L-cysteine 0.3
Sodium sulphite 0.2
pH: 7.3 ± 0.2
Directions
Suspend 37.5 grams of powder, disperse in 1 litre of deionised water. Allow to stand for 10 minutes, swirl to mix then sterilise by autoclaving at 121șC for 15 minutes. Allow to cool to 80șC, add 50ml of sterile horse blood and allow to "chocolate". Further cool to 47șC before adding antibiotic selective agents. Mix well and pour into petri dishes.
The medium is a sugar free base with a mixture of casein and soy peptones as nutrients and with L-cystine and sodium sulphite as supplements and reducing agents. The medium is made selective with the addition of amphoteracin (5 mg/l) and trimethoprim (10 mg/l). Streptomycin (200 mg/l) can also be used but sensitive variants of T. equigenitalis have been described.
Q.C. organisms:
T.equigenitalis
E.coli (inhibition)
Storage: up to 7 days at 2-8șC in the dark.
Inoculation: Surface, streaking out for single colonies.
Incubation: 37șC in 10% CO2 for 2-3 days.
References
Atherton, J. G. 1978. Inhibition of the C.E.M. organism in mixed cultures. Vet. Rec. 432.