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CAMPYLOBACTER BLOOD FREE MEDIUM BASE (KARMALI)

Code: CB1/2832

Typical formula g/l

Peptone mix 10.0
Tryptose 10.0
Peptone 3.00
Maize starch 1.00
Sodium chloride 5.00
Charcoal 4.00
Hematine 0.032
Sodium pyruvate

0.10

Cyclohexamide 0.10
Agar 14.0
Tris 1.0

pH 7.4 +/- 0.2

Directions

Suspend 24.1g of Campylobacter Blood Free Medium Base Karmali in 500 ml of distilled water; heat to boiling with agitation and autoclave at 115°C for 15 minutes. Cool to 50°C and add the contents of one vial of Karmali Supplement. Distribute into sterile petri dishes with frequent stirring.

Quality assurance (48 h-42°C, reduced O2)

Productivity control

C. jejuni ATCC 33291*: growth

Selectivity control

E. coli ATCC 25922*: partially or completely inhibited

*NCCLS M22-A2 recommended strains

Storage

Dehydrated media: 15-30°C

User prepared plates and tubes: up to 7 days at 2-8°C

References

Bolton, F.J., Hutchinson, D.N., Coates, D. (1986) J. App. Bacteriol. 56, 151-157

Bolton, F.J., Robertson, L. (1982) J. Clin. Pathol. 35, 462-467

Karmali, M.A., Simor, A.E., Roscoe, M., Fleming, P.C., Smith, S.S., Lane, J. (1986) J. Clin. Microbiol. 21, 456-459

NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-Second Ed.; Approved Standard

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