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CAMPYLOBACTER ENRICHMENT BROTH

A selective enrichment broth for the isolation of Campylobacter spp. from food, environmental samples and faeces.

Code: CB1/1203

Typical formula g/l

Meat peptone

10.0

Lactalbumin hydrolsates 5.0
Yeast extract 5.0
Sodium chloride 5.0
Haemin 10.0mg
Sodium pyruvate 0.5
Ketoglutaric acid 1.0
Sodium metabisulphite

0.5

Sodium carbonate 0.6

pH: 7.4 ± 0.2

Directions

Suspend 27.6g of powder, disperse in 1 litre of deionised water and allow to stand for 10 minutes. Swirl to mix and autoclave at 121șC for 15 minutes. Cool to 47șC, add 2 vials of C.T.V.C. selective supplement (reconstituted with 5mls of 50% alcohol) and 50 mls of saponin lysed horse blood, mix well and dispense into sterile containers.

Description

The use of a selective enrichment broth enhances the recovery of sub-lethally damaged organisms due to processing of foods, or if small numbers of campylobacters are present in heavily contaminated specimens.

This broth has been shown to give appreciably better results than Preston Broth.

Q.C. organisms:

Campylobacter jejuni

E.coli (inhibition)

Storage: Capped containers: 7 days at 2-8șC in the dark.

Inoculation: Food homogenate is added to broth in a ratio of 1:4 (w/v) in screw cap containers leaving a head space of 1.5 cm. For faeces 1 ml of a 10% suspension in Buffered Peptone Water is added to 5 ml of broth.

Incubation: Aerobically at 37șC for 2-4 hours, followed by a further 14-48 hours at 42șC.

Subculture: On Campylobacter Blood Free Selective Medium.

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