CAMPYLOBACTER BLOOD FREE MEDIUM BASE
Code: CB1/1017
Typical formula g/l
| Beef extract | 10.00 |
| Peptone | 10.00 |
| Tryptone | 3.00 |
| Sodium chloride | 5.00 |
| Charcoal | 4.00 |
| Sodium desoxycholate | 4.00 |
| Ferrous sulphate | 1.00 |
| Sodium pyruvate | 0.25 |
| Agar | 15.00 |
pH 7.4 +/- 0.2
Methods
CBFMB PLATING MEDIUM; suspend 45g of Campylobacter Blood Free Medium Base in 1000ml of distilled water; heat to boiling with agitation and autoclave at 121°C for 15 minutes. Cool to 50°C and add the contents of one vial of Cefoperazone Supplement. Distribute into sterile petri dishes with frequent stirring.
Quality assurance (48 h-42°C, reduced O2)
Productivity control
C. jejuni ATCC 33291*: growth
Selectivity control
E. coli ATCC 25922*: partially or completely inhibited
*NCCLS M22-A2 recommended strains
Storage
Dehydrated media: 15-30°C
User prepared plates and tubes: up to 7 days at 2-8°C
References
Bolton, F.J., Hutchinson, D.N., Coates, D. (1986) J. App. Bacteriol. 56, 151-157
Bolton, F.J., Robertson, L. (1982) J. Clin. Pathol. 35, 462-467
Karmali, M.A., Simor, A.E., Roscoe, M., Fleming, P.C., Smith, S.S., Lane, J. (1986) J. Clin. Microbiol. 21, 456-459
NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-Second Ed.; Approved Standard