BUFFERED SODIUM ENRICHMENT PEPTONE SOLUTION
A sample preparation liquid medium.
Code: CB1/3952
Typical formula g/l
| Potassium dihydrogen phosphate | 3.60 |
| Disodium phosphate (anhydrous) |
5.76 |
| Sodium chloride | 4.30 |
| Pancreatic digest of casein |
1.00 |
pH 7.0 +/- 0.2
Directions
Suspend 14.6g in 1000ml of cold distilled water. Heat to dissolve, distribute into tubes or flasks and sterilise by autoclaving at 121ºC for 15 minutes. Before sterilisation surface-active agents or inactivators of antimicrobial agents may be added, such as Polysorbate 80, 1g/ltr to 10g/ltr. A typical neutralising fluid is supplemented before sterilisation with: Polysorbate 80 30g/ltr, Lecithin 3g/ltr, histidine HCl 1g/ltr.
Description
Buffered sodium chloride-peptone solution is prepared according to the formulation recommended by European Pharmacopoeia. It is used as a universal diluent for sample preparation used in the microbiological examination of non-sterile products. Refer to EP 3rd edition for details of the procedures of sampling and sample preparation.
Storage
Dehydrated medium: 15-30°C
User prepared tubes: 3 months at 2-8°C