BRUCELLA BROTH

For the cultivation of Brucella and other fastidious and non-fastidious microorganisms.

Code: CB1/3742

Typical formula g/l

Peptone	20.0
Yeast extract	2.0
Glucose	1.0
Sodium chloride	5.0
Sodium bisulphite	0.1
Sodium phosphate	3.0

pH 7.0 +/- 0.1

Directions

Suspend 31.1g in 1000 ml of cold distilled water. Heat to dissolve, distribute and sterilise by autoclaving at 121°C for 15 minutes.

Description

Brucella Broth is a high nutritive solid medium used for the cultivation of Brucella and other fastidious and non-fastidious microorganisms. Brucella Broth is used as basal medium for the isolation of Campylobacter and other fastidious microorganisms.

Method

Inoculate the medium with purified cultures or other type of inoculum. For the cultivation of Brucella incubate the inoculated tubes for up to 7 days at 37^{_{°C in an aerobic atmosphere with carbon dioxide. For the cultivation of other microorganisms, incubate at the required optimum temperature in a suitable atmosphere for the time necessary to develop growth.}}

_Storage

_{Dehydrated medium: 15-30°C}