BRILLIANT GREEN BILE (2%) BROTH with MUG

A liquid medium for the detection of coliforms and E.coli.

Code: CB1/3662

Typical formula g/l

Ox bile	20.00
Lactose	10.00
Peptone	10.00
Brilliant green	0.0133
MUG	0.100

pH 7.2 +/- 0.2

Directions

Suspend 40g in 1000 ml of cold distilled water; heal to dissolve, distribute into fermentation tubes and sterilise by autoclaving at 121^{_{°C for 15 minutes. Do not exceed time and temperature of sterilisation. With inocula greater than 1ml for 10ml of medium, use} multiple strength medium.}

Description

_{Brilliant Green Bile 2% Broth MUG is used for the detection of E. coli, with the MPN procedure, in water and foods. Incubate the inoculated tubes for 18-24 hours and observe periodically for the development of fluorescence using a Wood's lamp. Refer to the various compendia for the test being performed and to technical sheet of Brilliant Green Bile 2% Broth for the details of the procedure for the detection and confirmation of coliforms.}

User quality assurance (37°C - 24 hrs)

Productivity control

E.coli ATCC 25922: growth, gas production, fluorescent under Wood’s lamp

C. freundii ATCC 43864: growth, gas production, not fluorescent under Wood’s lamp

Selectivity control

E. faecalis ATCC 19433: partially inhibited, no gas production

Storage

Dehydrated medium: 15 - 30°C

User prepared plates: 1 month at 2 - 8°C in the dark