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BLOOD AGAR BASE (2)

 

An improved blood agar base for the cultivation of fastidious and non-fastidious microorganisms.

 

Code: CB1/1008

 

Typical formula g/l

 

Peptone 15.0
Liver extract

2.5

Yeast extract 5.0
Sodium chloride

5.0

Agar

13.0

 

Final pH 7.4 +/- 0.1

 

Directions

Suspend 40.5 g in 1000 ml of cold distilled water, heat to boiling and autoclave at 121°C for 15 minutes. Cool to 45-50°C and add 5-7% sterile defibrinated blood.

 

Description

Blood Agar Base No. 2 is a modification of Blood Agar Base studied in order to obtain a superior growth of fastidious microorganisms. The peptones present in the medium allow achieve accurate haemolytic zones and an increase in the size of the colonies.

 

User quality assurance (Blood Agar Base no. 2 + 5% def. blood) (37°C-24 h)

 

Productivity control

S.pyogenes ATCC 19615: good growth, β haemolysis

S.pneumoniae ATCC 6305: good growth, α haemolysis

S.aureus ATCC 25923: good growth

E.coli ATCC 25922: growth

 

Storage

Dehydrated media: 15-30°C

User prepared plates: 1 month at 2-8°C

 

Reference

NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-2nd ed.; Approved Standard.

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