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BLOOD AGAR BASE (I)

 

Basal medium for the preparation of blood agar plates.

 

Code: CB1/1007

 

Typical formula g/l

 

Beef extract 10.0
Tryptose 10.0
Sodium chloride 5.0
Agar 12.0

 

    

Directions

Suspend 37 g in 1000 ml of cold distilled water, heat to boiling and autoclave at 121ºC for 15 minutes. Cool to about 50°C, aseptically add 5% (v/v) of sterile defibrinated blood; mix well and pour into sterile petri dishes. Incubate at 37°for at least 24 hours before use, to test the sterility.

Final pH 7.4 +/- 0.2

 

Description

Blood Agar Base is a multipurpose medium suita­ble for the growth of pathogenic and non-pathogenic microorganisms. With defibrinated animal blood the medium is suitable for the preparation of blood agar plates for the cultivation of streptococci, staphylococci, pneumococci and for the determination of their haemolytic acti­vity.  With blood agar horse H.influenzae develops a beta haemolytic halo and mimics S.pyogenes. With serum or other enrichments Blood Agar Base, is suitable for the cultivation of fastidious microorganisms.

Without any addition the medium is used as a rich nutrient medium.

 

User quality assurance

(Blood Agar Base + 5% def. blood)(37°C-24hrs)

 

Productivity control

S.pyogenes ATCC 19615*: good growth, β haemolysis

S.pneumoniae ATCC 6305*: good growth, α haemolysis

S.aureus ATCC 25923*: good growth

E.coli ATCC 25922*: growth

*NCCLS M22-A2 recommended strains.

 

Storage

Dehydrated media: 15-30°C

User prepared plates: 1 month at 2-8°C

 

Reference

NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-2nd ed.; Approved Standard. 

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