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BRAIN HEART INFUSION BROTH 

 

A general purpose liquid medium in powder and ready to use, for the cultivation of fastidious and non-fastidious microorganisms.

Code: CB1/1011

 

Typical formula g/l

 

BHI solids  

12.2

Peptone mixture

15.0

Glucose 2.0
Sodium chloride

5.0

Disodium phosphate 2.5

 

Final pH 7.4 +/- 0.2   

 

Directions

Suspend 37g of BHI broth in 1000 ml of cold distil­led water. Heat to boiling, distribute and sterilise by autoclaving at 121°C for 15 minutes.

 

Description

Brain Heart infusion Broth is used for the cultivation of a wide variety of microorganisms, including fastidious orga­nisms, moulds and yeasts. With the addition of penicillin (20 I.U./ml) and streptomycin (40 I.U/mI) the media become selective for pa­thogenic fungi, the growth of bacteria and many saprophytic fun­gi, being retarded and/or inhibited. The addition of paraminobenzoic acid, which neutralises sulpho­namides facilitates the isolation of microorganisms from patients undergoing chemotherapeutic treatment. Brain Heart Infusion Broth is especially recommen­ded for the cultivation of streptococci, pneumococci and menin­gococci, and for the preparation of Castaneda biphasic blood-culture bottles. With the addition of 10% horse blood and the agar concentration of 2%, BHI Broth is recommended for the preparation of the culture of S.aureus to be used for coagulase test.

 

User quality assurance (24 h -37°C)

 

Productivity control

S.aureus ATCC 25923: growth

E.coli ATCC 25922: growth

 

Storage

Dehydrated media: 15-30°C

User prepared plates and tubes: 1 month at 2-8°C

 

Reference

NCCLS document M22-A2, 1996. Quality Assurance for Commercially prepared Microbiological Culture Media-2nd ed.; Approved Standard.

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