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BACILLUS CEREUS MEDIUM

For the enumeration of Bacillus cereus in foods.

Code: CB1/1005

Typical formula g/l

Beef Extract 1.0
Peptone 10.0
D-Mannitol

10.0

Sodium chloride 10.0
Phenol Red 0.025
Agar 15.0

pH: 7.2 ± 0.2

Directions

Weigh 46 grams of powder, disperse in 900ml of deionised water. Allow to stand for 10 minutes, swirl to mix then sterilise by autoclaving at 121șC for 15 minutes. Cool to 47șC and aseptically add 100 mls of egg yolk emulsion and 2 vials of Polymixin Selective supplement.

Q.C. organisms:

B.cereus

E.coli (inhibition)

Storage: up to 7 days at 2-8șC in the dark.

Inoculation: Surface, spreading or streaking for single colonies.

Incubation: 30șC aerobically for 24-48 hours.

References

Mossel, D. A. A., Koopman, M. J. and Jongerius, E. 1967. Enumeration of Bacillus cereus in foods. Appl. Microbiol. 15: 650-653. Thatcher, F. S., Clarke, D. S. 1978 Micro-organisms in foods. Volume 1 second edition. University of Toronto.

BS5763 Part 1L:1994. ISO7932:1993 3/100

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