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AZIDE VIOLET BLOOD AGAR BASE

 

A highly selective medium for the isolation of Streptococci.

 

Code: CB1/3102

 

Typical Formula g/l

 

Tryptose 10.00
Beef extract 3.00
Sodium chloride 5.00
Sodium azide 0.20
Crystal violet 0.002
Agar 15.00

      

Directions

Suspend 33.2 g in 1000 ml of cold distilled water, heat to boiling distribute and sterilise by autoclaving at 121°C for 15 minutes. To prepare blood agar plates cool to about 50°C, aseptically add 5%(v/v) of sterile defibrinated blood, mix well and pour into sterile petri dishes.

 

WARNING: see hazardous precautions for media containing azide.

 

Description

Azide Violet Blood Agar Base, with sodium azide and crystal violet, is highly selective for streptococci, with Gram-negative organisms and staphylococci being inhibited. Azide Violet Blood Agar Base is recommended for the isolation of enterococci in urine and is suggested by Mossel for the enu­meration of faecal streptococci in foodstuffs.

 

User quality assurance (37°C-24 h)

 

Productivity control

S.pyogenes  ATCC 19615: growth, beta haemolysis

S.pneumoniae ATCC 6305: growth alpha haemolysis

 

Selectivity control

S.aureus ATCC 25923: inhibited

E.coli ATCC 25922: inhibited

 

Storage

Dehydrated medium: 15-30°C

User prepared plates: up to 24 hours at 2-8°C

 

Reference  

Mossel, D.A.A., Diepen, H.M.S De Bruin, A.S. (1967). J. Appl. Bact. 20, 265-272

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