ASPARAGINE ENRICHMENT BROTH

A selective medium for the detection of Pseudomonas aeruginosa.

pH 7.0+/- 0.2

Suspend 4.5g in 1000ml of cold distilled water: heat to dissolve, distribute and sterilise at 121^{_{°C for 15 minutes. Inoculate not more than 1ml sample in 10 ml of medium or use a multiple strength medium.}}

_{Asparagine Enrichment Broth is recommended by APHA as a selective medium for the detection of Pseudomonas aeruginosa in pool and bathing waters.}

_{Collect the specimens to be tested in bottles containing sodium thiosulfate (about 100mg per litre of specimen) in order to neutralise the possible residual chlorine. Inoculate five 10ml, five 1ml and five 0.1ml samples into Asparagine Enrichment Broth. Examine for the presence of P.aeruginosa according to the most probable number method, counting as positive all the tubes where presence of a green-blue fluorescent pigment (under long-wave ultraviolet light), and turbidity are observed after incubation at 35°C for 48 hours. The count obtained in this way is to be considered as presumptive.}

_{User quality assurance (37°C-24 hrs)}

_{P.aeruginosa ATCC 27853: good growth}

_{E.coli ATCC 25922: partially inhibited}

_{APHA (1985) - Standard Methods for the Examination of Water and Wastewater, 16th edition, pp. 978-980.}