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ANTIBIOTIC BROTH A 3

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A nutrient broth for the turbidimetric assay of antibiotics.

 

Code: CB1/3651

 

 

Typical formula g/l

 

Peptone 5.00
Yeast extract 1.50
Beef extract 1.50
Sodium chloride 3.50 
Glucose 1.00
Dipotassium phosphate 3.68
Monopotassium phosphate 1.32

 

Final pH 7.0 +/- 0.1

 

Directions

 

Suspend 17.5 g in 1000 ml of cold distilled water; heat to dissol­ve, distribute and sterilise by autoclaving at 121°C for 15 minutes. The medium in prepared according to the specifications given in FDA, 21 CFR and in USP XXIV Ed. It corresponds to Medium no. 3 of Grove and Randall: nutrient broth for the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus.

 

Storage

 

Dehydrated medium: 15-30°C

User prepared flasks: 1 month at 2-8°C

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