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ANTIBIOTIC BASE AGAR

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A base agar for the microbiological assay of antibiotics.

 

Code: CB1/3501

 

Typical formula g/l

 

Peptone 6.0
Yeast extract

3.0

Beef extract

1.5

Agar 15.0

 

 

Final pH 6.6 +/- 0.1

 

Directions

Suspend 25.5 g in 1000 ml of cold distilled water; heat to boiling and sterilise by autoclaving at 121°C for 15 minutes. The medium is prepared according to the specifications given in FDA, 21 CFR and in USP XXIV Ed. It corresponds to Medium no.2 of Grove and Randall: base agar for the plate assay of antibiotics. It is used as the base agar in the microbiological assay of bacitracin and penicillin G.

 

Storage

Dehydrated medium: 15-30°C

User prepared flasks: 1 month at 2-8°C

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