ANTIBIOTIC AGAR A 1
A seed layer agar for microbiological assay of antibiotics.
Code: CB1/8752
Typical formula g/l
| Peptone | 6.0 |
| Tryptone | 4.0 |
| Yeast extract | 3.0 |
| Beef extract | 1.5 |
| Glucose | 1.0 |
| Agar | 15.0 |
pH 6.5 +/- 0.1
Directions and Description
Suspend 80.5 g in 1000 ml of cold distilled water; heat to boiling and sterilise by autoclaving at 121°C for 15 minutes. The medium is prepared according to the specifications given in FDA, 21 CFR and in USP XXIV ed. It corresponds to Medium no. 1 of Grove and Randall: seed agar for microbiological assay of antibiotics and maintenance agar for test strains.
It is used with M.flavus for the plate assay of bacitracin, with Sarcina lutea for the assay of chloramphenicol and with S.aureus for the assay of kanamycin sulphate, penicillin G, sodium methicillin and sodium oxacillin. It is also used as a base agar in the assay of the following antibiotics: chloramphenicol, kanamycin, colistin, methicillin, oxacillin, and vancomycin.
Storage
Dehydrated medium: 15-30°C
User prepared flasks: 1 month at 2-8°C