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ANAEROBE LIQUID MEDIUM

Designed to give optimum growth of demanding anaerobes.

Code: CB1/1001

Typical formula g/l

Peptone mix

15.0

Yeast extract 10.0
L-Cysteine HCl 0.5
Resazurin 0.001
Sodium bicarbonate 0.4
Haemin 0.005
Vitamin K 0.0005
Sodium thioglycollate

0.5

Sodium chloride 2.5
Agar 0.75

pH 7.2 ± 0.2

Directions:

Suspend 30 grams of powder, disperse in 1 litre of deionised water. Allow to stand for 10 minutes, swirl to mix. Boil to dissolve the agar then dispense into screw cap containers. Sterilise by autoclaving at 121șC for 15 minutes. Tighten the caps as soon as possible after autoclaving. Do not reheat more than once.

Growth -indicators:

The broth may become turbid or individual colonies may form suspended in the medium

Description:

The medium was designed to give optimum growth of anaerobes. It is rich in nutrients from the peptone content in the formulation. Vitamin K. haemin and L-cysteine are growth factors required by some anaerobes. L-cysteine together with sodium thioglycollate reduce the Eh of the medium and the agar content inhibits absorption of oxygen and convection currents. Resazurin is a redox indicator.

Q.C. organisms: B.fragilis

Storage: Capped containers up to 3 months at 15-20șC in the dark.

Inoculation: If used as a blood culture medium a minimum dilution of 1:10 should be used.

Incubation: 37șC for 24-72 hours. Keep the container airtight

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