[an error occurred while processing this directive] [an error occurred while processing this directive] [an error occurred while processing this directive]

ACETATE DIFFERENTIAL AGAR

For the differentiation of Shigella species from Escherichia coli and for the differentiation of non-fermenting Gram-negative bacteria.

Code: CB1/6802

Typical formula  g/l

Sodium acetate 2.0
Sodium chloride 5.0
Magnesium sulphate 0.2
Dipotassium phosphate 1.0
Agar 20.0
Bromothymol Blue 0.08
Ammonium dihyd. phos. 1.0

pH 6.8 +/- 0.2

Directions

Weigh 29.3 grams of powder and add to 1 litre of deionised water (conductivity <10ms). Gently heat and bring to boiling. Distribute into tubes to produce a 1cm butt and 30cm slant. Autoclave for 15 minutes at 121°C (equivalent heat process). Cool tubes in slanted position.

Description

Used for the differentiation of Shigella species from Escherichia coli and also for the differentiation of non-fermenting Gram-negative bacteria. Bacteria that can utilise acetate as the sole carbon source turn the medium blue.

Storage

Dehydrated medium: 15-30°C

User prepared tubes: 7 days at 2-8°C

References

• Simmons, J.S. (1926) - J. Infect. Dis. 39, 209-214.

[an error occurred while processing this directive]