EE BROTH

 

Liquid culture medium for enrichment of enterobacteria from food samples.

 

Code: CB1/1029

 

Typical formula g/l

 

Gelatin peptone	10.00
Dextrose	5.00
Ox bile	20.00
Di-sodium phosphate	6.45
Monopotassium phosphate	2.00
Brilliant green	0.015

 

Final pH 7.2 ± 0.2

 

Directions

Suspend 43.5 g of powder in 1 litre distilled water and heat until dissolution. Sterilise at 121°C for 5 minutes or boil it for 30 minutes. Cool quickly.  OVERHEATING THIS MEDIUM WILL ADVERSELY AFFECT ITS PERFORMANCE.

 

Description

This medium is for enterobacteria enrichment and it is a Mossel’s modification (1963) to the classic Brilliant Green Bile 2% Broth. Substitution of lactose by glucose makes it more suitable for enteric bacteria detection, even gas producer or non-gas-producer, in foods and different samples. The recommended technique is as follows: sample to study is added to sterile broth at 10% proportion. After a strong homogenisation the mixture is incubated for 18-20 hours period at 35-37°C. Following, subcultures are performed on a solid media appropriate to the selective enterobacteria isolation. For this step, it is especially suggested Violet Red Bile Agar though there are also the MacConkey, Desoxycholate or Brilliant green based media. For suspicious colonies on this media identification will be performed following the most common methodology.

 

References

MOSSEL, VISSER and CORNELISSEN (1973) J. Appl.Bact. 26:444

CeNAN (1976) Métodos de Exámen Microbiologico para alimentos y Bebidas. Madrid.

PASCUAL ANDERSON, MªRª. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.