Contents: Scroll down the page to browse the products available for the laboratory diagnosis of TB
Culture media
Lowenstein-Jensen Medium. An egg-potato base solid media containing malachite green (an inhibitory agent). The use of L-J media is excellent for the recovery of M. tuberculosis from sterile-site specimens as well as decontaminated-digested sputum specimens.
- Supplied as 500g dehydrated culture medium (CB2/1116), pre-poured slopes in 50 x 7ml universals (CB39/0200), 25 x 7ml heavy universals (CB39/0200) and 25 x 5ml heavy bijous (CB39/0200)
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Lowenstein-Jensen Pyruvate Medium. Pyruvate is an intermediary organic acid metabolite in glycolysis and the first of the Embden Myerhoff pathway that can pass readily into or out of the cell. Thus, its addition to culture medium provides both an energy source and a carbon skeleton for anabolic processes. Its addition may help in maintaining certain and may help when when the serum concentration is reduced in the medium. Pyruvate in LJ medium encourages the growth of M. bovis.
- Supplied as pre-poured slopes in 50 x 7ml universals (CB39/0210), 25 x 7ml heavy universals (CB39/0210) and 25 x 5ml heavy bijous (CB39/0210)
Acid Egg Medium. The least expensive of all TB-specific media available and supports good growth of tubercle bacilli. Contamination during preparation is limited because it is inspissated after being placed in bottles. In addition, the malachite green added to the media suppresses the growth of non-mycobacterial organisms
- Supplied as pre-poured slopes in 25 x 7ml heavy universals (CB39/0010)
Pyruvic Acid Egg Medium.
- Supplied as pre-poured slopes in 50 x 7ml universals (CB39/0260), 25 x 7ml heavy universals (CB39/0260) and 25 x 5ml heavy bijous (CB39/0260)
Kirchner Liquid Medium. A liquid culture medium which is made selective by the addition of antibiotics and has been shown to be sufficient in the recovery of mycobacteria from non-sputum samples.
- Supplied as 25 x 10ml heavy universals (CB39/0180)
Stains
Ziehl-Neelsen. Staining according to the Ziehl-Neelsen reference method for acid-fast bacilli.
- Individual stain for ZN available: Ziehl carbol fuchsin as 125ml, 500ml, 1000ml and 2500ml (CB28/320490)
- Individual stain for ZN available: Carbolic Methylene Blue as 125ml, 1000ml and 2500ml (CB28/310100)
Kit BK-Colour. The Ziehl-Armand stain is a fast variation of the traditional ZN stain and gives the advantage of simultaneously decolourising all bacteria except mycobacteria that have taken up the carbol fuchsin stain.
- Supplied as Kit BK-Colour: 1 kit = 1 x 100ml of Ziehl carbol fuchsin and 1 x 100ml of Armand solution, providing sufficient reagents for 125 to 200 slides (CB28/364600).
- Individual stain for Kit BK-Colour available: Ziehl carbol fuchsin as 125ml, 500ml, 1000ml and 2500ml (CB28/320490)
- Individual stain for Kit BK-Colour available: Armand Solution as 125ml and 1000ml (CB28/360100)
Cold AFB stain. This cold AFB stain offers the advantage of not having to heat slides and producing toxic carbolic fumes. The stain is very quick and suitable on all types of substrates (sputa, cultures, etc). Sensitivty is equal to that of traditional ZN staining. Mycobacteria appear pink against the blue background of the slide.
- Supplied as Kit Quick-TB: 1 kit = 1 x 125ml of Carbol fuchsin RAL and 1 x 125ml of Armand Soultion, providing sufficient reagents for 125 to 200 slides (CB28/361560).
- Individual stain for Kit Quick-TB available: Carbolic fuchsin RAL as 1000ml (CB28/365240)
- Individual stain for Kit Quick-TB available: Armand Solution as 125ml, 500ml and 1000ml (CB28/360100)
Kit Quick-TB
Fluorescent Auramine stain. Fluorescent staining according to the Degommier is a fast technique that helps detect AFBs. Stained AFBs appear fluoresent yellow-green against a red background of the slide. Fluorescent staining allows fast screening and easy reading of slides. It is essential to confirm fluorescent-positive AFB by staining the same slide with a Ziehl technique.
- Supplied as Kit Fluo-Color: 1 kit = 1 x 250ml of Carbolic auramine, 1 x 250ml Degommier discolouring solution, 1 x 125ml Thiazine red concentrated solution and 1 x 125ml Carbolic solution (CB28/361460).
- Individual stain for Kit Fluo-Color: Carbolic auramine as 500 and 1000ml (CB28/361430)
- Individual stain for Kit Fluo-Color: Degommier discolouring solution as 1000ml (CB28/320800)
- Individual stain for Kit Fluo-Color: Thiazine red concentrated solution as 500ml (CB28/320780)
- Individual stain for Kit Fluo-Color: Carbolic solution as 500ml (CB28/320790)
Kit Fluo-Color
Phage-based Assay for TB Detection
A novel application of phage amplification technology, which allows the rapid detection of TB within 24 hours of sample preparation. The technique involves infecting mycobacterial cells present in the sample with a specific mycobacteriophage then treating the sample with a virucide to destroy any extracellular mycobacteria. Intracellular phage undergo replication and are released from host cells into a lawn of susceptible cells within an agar plate. The phage undergo further replication to produce plaques on the surface of the agar, indicating the presence of TB.
- Supplied as:
- FastPlaque TB™ Kit. 50 tests (CB30/5100)
FastPlaque TB™ Kit
Phage-based Assay for Detection of Rifampicin-Resistant TB
Extension of the FastPlaque TB™ Kit (above) allows early detection of multi-drug resistant tuberculosis. A smear-positive sputum specimen is processed in a similar manner to the FastPlaque TB™ assay but preincubated overnight with and without rifampicin. Viable TB bacilli in the original specimen are reflected as plaques in the lawn of sensor cells. Rifampicin-resistant TB strains survive drug treatment and produce plaques, whilst sensitive strains do not.
Supplied as:
- FastPlaque Response TB™ Kit. 25 tests (CB30/5200)
- Fast Plaque NOA Antimicrobial Supplement . 5 vials, sufficient for one FastPlaque Response Kit, i.e.25 tests (CB30/5250)
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Microscopes
Cosmos Biomedical Ltd supply a range of microscopes for diagnostic and reseach laboratory use. We source these instruments from Kozo Optics. Find out more about these microscopes here: http://www.kozo-optics.com/
Microscope Immersion Oil
Immersion Oil is important to fine image resolution and brightness. These factors are most critical under high magnification so only higher power (generally 40 to 120x), short focus objectives are designed for use with immersion oil.
- Supplied as:
- Pack Immersion 100 (10ml x 10ml) (CB76/340100)
- Pack Immersion 150 (6 x 25ml) (CB76/340150)
- Pack Immersion 300 (6 x 50ml) (CB76/340300)
Sputum & Urine Containers
Polycon® containers are sterile shatterproof polypropylene specimen container with flat or conical base for transport of clinical specimens for laboratory analysis. Shatterproof container for safe handling and transport. Container made from virgin polypropylene, chemically inert, does not affect specimen. Cap features integral 7-layer non-permeable liner resistant to acids, alkalis and alcohols.
- Supplied as:
- Polycon® 27ml. Unlabelled (pack size = 500) (CB31/205)
- Polycon® 27ml. Printed label (pack size = 500) (CB31/206)
- Polycon® 27ml. Plain Label (pack size = 500) (CB31/206PL)
Polycon® 27ml, printed label
- Polycon® 60ml Unlabelled (pack size = 300) (CB31/270)
- Polycon® 60ml Printed label (pack size = 300) (CB31/271)
- Polycon® 60ml Plain Label (pack size = 300) (CB31/271P)
Polycon® 60ml printed label
- Polycon® 110ml Unlabelled (pack size = 160) (CB31/290)
- Polycon® 110ml Printed label (pack size = 160) (CB31/291)
- Polycon® 110ml Plain label (pack size = 160) (CB31/291PL)
Disposable Inoculating Loops & Swabs
Sterile disposable plastic inoculating loops for precise volumetric sampling and transfer of material for accurate analysis. Accurate sampling, consistent and reproducible results. Precision moulded – no gouging of media. No need for Bunsen and no aerosols.
- Supplied as:
- Microloops® D 10μl. Individually packed. Pack size = 1000 (CB31/700/1)
- Microloops® D 10μl Packed in 20 per sleeve. Pack size = 1000 (CB31/700/20)
- Microloops® D 1μl Individually packed. Pack size = 1000 (CB31/701/1)
- Microloops® D 1μl Packed in 20 per sleeve. Pack size = 1000 (CB31/701/20)
Microloop® D 1μl
- Supplied as:
- Dryswab ™ Woodstick shaft in peel pouch. Pack size = 1250 (CB31/108)
- Dryswab ™ Woodstick shaft in peel pouch. Pack size = 300 (CB31/108/10)
- Dryswab ™ Woodstick shaft in peel pouch. Pack size = 600 (CB31/108/2)
- Dryswab ™ Woodstick shaft in peel pouch. Pack size = 100 (CB31/108/20)
- Dryswab ™ Woodstick shaft in peel pouch. Pack size = 500 (CB31/108/5)
- Dryswab ™ Woodstick shaft bulk packed. Ppack size = 5000 (CB31/116)
- Dryswab ™ Woodstick shaft bulk packed. Sterile. Pack size = 5000 (CB31/116S)
Dryswab ™ Woodstick shaft